serca2a (Cell Signaling Technology Inc)

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Cell Signaling Technology Inc serca2a
Serca2a, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 87 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+serca2/ATP2A2%2FSERCA2+Rabbit+mAb/pm41707812-100-23-24
Average 94 stars, based on 87 article reviews

serca2a - by Bioz Stars, 2026-09

94/100 stars

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Immunodetection:

Article Title: Disruption of Sarcoplasmic Reticulum‐Mitochondrial Contacts Underlies Contractile Dysfunction in Experimental and Human Atrial Fibrillation: A Key Role of Mitofusin 2
Article Snippet: Next, protein homogenates were separated by 4% to 20% TGX gels (Bio- Rad), transferred to nitrocellulose membranes. .. Subsequently, immunodetection was done by incubation with primary antibodies: anti- MFN2 (1:1000, Cell Signaling, 9482), anti- MFN1 (1:500, Abcam, ab104274), anti- SERCA2 (1:1000, Cell Signaling, 4388), anti- VDAC1 (1:1000, Abcam, ab15895), anti- TOM20 (1:1000, Santa Cruz, sc17764), and anti- GAPDH (1:25000, Fitzgerald, 10RG109A) overnight at 4 °C in a cold room, followed by a 3- time wash with 1% Tween 20 in TRIS- buffered saline solution, and incubation with horseradish peroxidase– conjugated anti- mouse or anti- rabbit secondary antibodies (Dako, 1:2000) for 1 hour at room temperature (RT). .. After washing with 1x TRIS- buffered saline solution, the intensity of the bands was quantified by using ImageQuant TL (GE Healthcare).

Article Title: Disruption of Sarcoplasmic Reticulum‐Mitochondrial Contacts Underlies Contractile Dysfunction in Experimental and Human Atrial Fibrillation: A Key Role of Mitofusin 2
Article Snippet: Next, protein homogenates were separated by 4% to 20% TGX gels (Bio‐Rad), transferred to nitrocellulose membranes. .. Subsequently, immunodetection was done by incubation with primary antibodies: anti‐MFN2 (1:1000, Cell Signaling, 9482), anti‐MFN1 (1:500, Abcam, ab104274), anti‐SERCA2 (1:1000, Cell Signaling, 4388), anti‐VDAC1 (1:1000, Abcam, ab15895), anti‐TOM20 (1:1000, Santa Cruz, sc17764), and anti‐GAPDH (1:25000, Fitzgerald, 10R‐G109A) overnight at 4 °C in a cold room, followed by a 3‐time wash with 1% Tween 20 in TRIS‐buffered saline solution, and incubation with horseradish peroxidase–conjugated anti‐mouse or anti‐rabbit secondary antibodies (Dako, 1:2000) for 1 hour at room temperature (RT). .. After washing with 1x TRIS‐buffered saline solution, the intensity of the bands was quantified by using ImageQuant TL (GE Healthcare).

Incubation:

Article Title: Disruption of Sarcoplasmic Reticulum‐Mitochondrial Contacts Underlies Contractile Dysfunction in Experimental and Human Atrial Fibrillation: A Key Role of Mitofusin 2
Article Snippet: HL‐1 cardiomyocytes were washed with PBS, incubated with 250 nmol/L Mitotracker Deep Red (Invitrogen) for 30 minutes at 37 °C, and washed with PBS. .. Then HL‐1 cardiomyocytes or RAA tissue samples of patients with SR or AF were fixed with 4% paraformaldehyde (Sigma) at RT for 15 minutes, washed 3 times with 1% Tween 20 in PBS (1x PBS‐T), blocked with 10% goat serum in PBS at RT for 60 minutes, incubated overnight at 4 °C with anti‐SEC61β (1:250, Thermo Fisher, PA3‐015), anti‐acetylated α‐tubulin (1:200, Sigma, T7451), anti‐SERCA2 (1:100, Cell signaling, 4388) or anti‐TOM20 (1:100, Santa Cruz, SC17764, only used for patient samples) primary antibodies diluted in in PBS with 1% BSA (1% PBSA), washed 3 times with 1× PBS‐T, incubated with donkey anti‐mouse (Alexa 488, Invitrogen) and anti‐rabbit (Alexa 555, Invitrogen) in 1% PBSA at RT in the dark for 1 to 2 hours, washed 3 times with 1× PBS‐T at RT, incubated with WGA Alexa 647 (1:50, Invitrogen) in PBS at RT 30 minutes, washed 3 times with 1× PBS‐T, incubated with DAPI (1:1000 in PBS, Invitrogen) at RT for 5 minutes, washed twice with 1× PBS‐T, then washed with PBS. .. The coverslips were mounted with DABCO (15 μL/sample, Invitrogen), and stored at 4 °C before microscopic analysis.

Article Title: Disruption of Sarcoplasmic Reticulum‐Mitochondrial Contacts Underlies Contractile Dysfunction in Experimental and Human Atrial Fibrillation: A Key Role of Mitofusin 2
Article Snippet: Next, protein homogenates were separated by 4% to 20% TGX gels (Bio‐Rad), transferred to nitrocellulose membranes. .. Subsequently, immunodetection was done by incubation with primary antibodies: anti‐MFN2 (1:1000, Cell Signaling, 9482), anti‐MFN1 (1:500, Abcam, ab104274), anti‐SERCA2 (1:1000, Cell Signaling, 4388), anti‐VDAC1 (1:1000, Abcam, ab15895), anti‐TOM20 (1:1000, Santa Cruz, sc17764), and anti‐GAPDH (1:25000, Fitzgerald, 10R‐G109A) overnight at 4 °C in a cold room, followed by a 3‐time wash with 1% Tween 20 in TRIS‐buffered saline solution, and incubation with horseradish peroxidase–conjugated anti‐mouse or anti‐rabbit secondary antibodies (Dako, 1:2000) for 1 hour at room temperature (RT). .. After washing with 1x TRIS‐buffered saline solution, the intensity of the bands was quantified by using ImageQuant TL (GE Healthcare).

Saline:

Article Title: Disruption of Sarcoplasmic Reticulum‐Mitochondrial Contacts Underlies Contractile Dysfunction in Experimental and Human Atrial Fibrillation: A Key Role of Mitofusin 2
Article Snippet: Next, protein homogenates were separated by 4% to 20% TGX gels (Bio‐Rad), transferred to nitrocellulose membranes. .. Subsequently, immunodetection was done by incubation with primary antibodies: anti‐MFN2 (1:1000, Cell Signaling, 9482), anti‐MFN1 (1:500, Abcam, ab104274), anti‐SERCA2 (1:1000, Cell Signaling, 4388), anti‐VDAC1 (1:1000, Abcam, ab15895), anti‐TOM20 (1:1000, Santa Cruz, sc17764), and anti‐GAPDH (1:25000, Fitzgerald, 10R‐G109A) overnight at 4 °C in a cold room, followed by a 3‐time wash with 1% Tween 20 in TRIS‐buffered saline solution, and incubation with horseradish peroxidase–conjugated anti‐mouse or anti‐rabbit secondary antibodies (Dako, 1:2000) for 1 hour at room temperature (RT). .. After washing with 1x TRIS‐buffered saline solution, the intensity of the bands was quantified by using ImageQuant TL (GE Healthcare).

Western Blot:

Article Title: Hypothalamic Thyroid Hormone Receptor α1 Signaling Controls Body Temperature.
Article Snippet: Background: The importance of thyroid hormones (THs) for peripheral body temperature regulation has been long recognized, as medical conditions such as hyperand hypothyroidism lead to alterations in body temperature and energy metabolism.. In the past decade, the brain actions of THs and their respective nuclear receptors, thyroid hormone receptor a1 (TRa1) and thyroid hormone receptor beta (TRb), coordinating body temperature regulation have moved into focus.. However, the exact roles of the individual TR isoforms and their precise neuroanatomical substrates remain poorly understood.

Whole Genome Amplification:

Article Title: Disruption of Sarcoplasmic Reticulum‐Mitochondrial Contacts Underlies Contractile Dysfunction in Experimental and Human Atrial Fibrillation: A Key Role of Mitofusin 2
Article Snippet: HL‐1 cardiomyocytes were washed with PBS, incubated with 250 nmol/L Mitotracker Deep Red (Invitrogen) for 30 minutes at 37 °C, and washed with PBS. .. Then HL‐1 cardiomyocytes or RAA tissue samples of patients with SR or AF were fixed with 4% paraformaldehyde (Sigma) at RT for 15 minutes, washed 3 times with 1% Tween 20 in PBS (1x PBS‐T), blocked with 10% goat serum in PBS at RT for 60 minutes, incubated overnight at 4 °C with anti‐SEC61β (1:250, Thermo Fisher, PA3‐015), anti‐acetylated α‐tubulin (1:200, Sigma, T7451), anti‐SERCA2 (1:100, Cell signaling, 4388) or anti‐TOM20 (1:100, Santa Cruz, SC17764, only used for patient samples) primary antibodies diluted in in PBS with 1% BSA (1% PBSA), washed 3 times with 1× PBS‐T, incubated with donkey anti‐mouse (Alexa 488, Invitrogen) and anti‐rabbit (Alexa 555, Invitrogen) in 1% PBSA at RT in the dark for 1 to 2 hours, washed 3 times with 1× PBS‐T at RT, incubated with WGA Alexa 647 (1:50, Invitrogen) in PBS at RT 30 minutes, washed 3 times with 1× PBS‐T, incubated with DAPI (1:1000 in PBS, Invitrogen) at RT for 5 minutes, washed twice with 1× PBS‐T, then washed with PBS. .. The coverslips were mounted with DABCO (15 μL/sample, Invitrogen), and stored at 4 °C before microscopic analysis.

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